p65 Antibody / NF-kB

• Catalog Number: orb2636857
• Category: Antibodies
• Description: Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor N B (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp B sequence in the immunoglobulin light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NF B is activated and NF B is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-RELA-1E3
• Tested applications: FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: Recombinant full-length human RELA protein was used as the immunogen for the p65 NF-kB antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This p65 NF-kB antibody is available for research use only.
• UniProt ID: Q04206
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the p65 NF-kB antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human MCF-7 cells using p65 NF-kB antibody (green, clone PCRP-RELA-1E3) and phalloidin (red).

FACS staining of PFA-fixed human K562 cells with p65 NF-kB antibody (blue, clone PCRP-RELA-1E3) and isotype control (red).

FACS staining of PFA-fixed human HeLa cells with p65 NF-kB antibody (blue, clone PCRP-RELA-1E3) and isotype control (red).

FACS staining of PFA-fixed human U-87 cells with p65 NF-kB antibody (blue, clone PCRP-RELA-1E3), and isotype control (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using p65 NF-kB antibody (clone PCRP-RELA-1E3). These results demonstrate the foremost specificity of the PCRP-RELA-1E3 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free p65 NF-kB antibody (PCRP-RELA-1E3) as confirmation of integrity and purity.