p63 antibody

• Catalog Number: orb2276313
• Category: Antibodies
• Description: p63 antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: Biorbyt-063R
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fragment (around aa600-680) of human TP63 protein (exact sequence is proprietary)
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: Q9H3D4
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: Amplified in squamous cell carcinoma (AIS); Chroni
• Note: For research use only
• Application notes: Positive Control: Tonsil: Virtually all squamous epithelial cells must show a moderate to strong, nuclear staining, while few scattered lymphocytes and endothelial cells must show a at least a weak staining. Negative Control: Tonsil: The vast majority of lymphocytes should be p63 negative. Cellular Localization: Nuclear Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Esophageal squamous cell carcinoma with intense p63 immunostaining of tumor cells.

In the esophagus a strong p63 staining occurs squamous epithelial cells.

Strong nuclear p63 positivity in basal cells of prostate glands.