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Catalog Number | orb1294319 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to p63 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rabbit |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / encompassing a sequence within the N-terminus region. |
Dilution range | Western Blot 1:500–1:1000 Immunofluorescence 1:100–1:300 Immunohistochemistry (Paraffin) 1:100 |
Conjugation | Unconjugated |
Target | p63 |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1%BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
All lanes: Anti-p63 antibody - at 1/500 dilution, Lysates/proteins at 50 μg per lane, This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA an hour before being incubated with orb1294319 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling p63 with orb1294319 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294319 anti-p63 antibody (red) at 1:100 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human tissue labeling p63 with orb1294319 at 1/100.
Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling p63 with orb1294319 at 1/100.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling p63 with orb1294319 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling p63 with orb1294319 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
ELISA, IF, IHC, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
FACS, IF, IHC-P, WB | |
Human, Mouse | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P, WB | |
Human, Mouse | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC, WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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