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Catalog Number | orb1151490 |
---|---|
Category | Antibodies |
Description | Rabbit monoclonal antibody to p63 |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | TP63/4379R |
Tested applications | IHC-P |
Reactivity | Human |
Isotype | Rabbit IgG, kappa |
Immunogen | A portion of amino acids 600-680 was used as the immunogen for the recombinant p63 antibody. |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml |
Purity | Protein A affinity |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This recombinant p63 antibody is available for research use only. |
UniProt ID | Q9H3D4 |
Storage | Aliquot the recombinant p63 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the recombinant p63 antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human prostate cancer with recombinant p63 antibody (clone TP63/4379R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate cancer tissue with recombinant p63 antibody (clone TP63/4379R). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate cancer tissue with recombinant p63 antibody (clone TP63/4379R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using recombinant p63 antibody (clone TP63/4379R). These results demonstrate the foremost specificity of the TP63/4379R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
ELISA, IF, IHC, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
FACS, IF, IHC-P, WB | |
Human, Mouse | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P, WB | |
Human, Mouse | |
Mouse | |
Monoclonal | |
Unconjugated |
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