p53 (phospho-Ser20) antibody

• Catalog Number: orb764257
• Category: Antibodies
• Description: Rabbit polyclonal antibody to p53 (phospho-Ser20)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human p53 around the phosphorylation site of Ser20. AA range:10-59
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 55
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-TP53 antibody, anti-P53 antibody, anti-Cellul
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using Phospho-p53 (S20) Polyclonal Antibody.

Western blot analysis of lysates from COS7 cells treated with UV 5', using p53 (Phospho-Ser20) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).