p53 Antibody / TP53

• Catalog Number: orb750107
• Category: Antibodies
• Description: Recognizes a 53 kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: TP53/1799R
• Tested applications: FACS, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Recombinant human p53 protein was used as the immunogen for this recombinant p53 antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Flow cytometry: 1-2ug/million cells
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant p53 antibody is available for research use only.
• UniProt ID: P04637
• Storage: Store the recombinant p53 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The optimal dilution of the recombinant p53 antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Protein array validation of the recombinant p53 antibody: Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using recombinant p53 antibody (clone TP53/1799R). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

IHC testing of FFPE human colon carcinoma with recombinant p53 antibody (clone TP53/1799R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

SDS-PAGE analysis of purified, BSA-free recombinant p53 antibody (clone TP53/1799R) as confirmation of integrity and purity.

Western blot testing of human HeLa cell lysate with recombinant p53 antibody (clone TP53/1799R).

Flow cytometry staining of human HeLa cells with recombinant p53 antibody (clone TP53/1799R); Red=isotype control, Blue=recombinant p53 antibody.