P2Y4 antibody

• Catalog Number: orb315641
• Category: Antibodies
• Description: Rabbit polyclonal antibody to P2Y4
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human P2Y4. The exact sequence is proprietary.
• Dilution range: WB: 1-500:1000, IF/ICC: 1-100:500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: P2RY4
• Entrez: 57385, 5030, 63843
• UniProt ID: Q9JJS7, O35811, P51582
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti NRU antibody, anti P2Y purinoceptor 4 antibod
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of P2Y4 expression in Hela (A), H460 (B), mouse lung (C), mouse brain (D), mouse kidney (E), rat kidney (F) whole cell lysates. (Predicted band size: 40 kD; Observed band size: 41 kD)

Immunofluorescent analysis of P2Y4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).