P2RX7 antibody

• Catalog Number: orb20291
• Category: Antibodies
• Description: Goat polyclonal antibody to P2RX7
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 3.75 μg/ml
• Conjugation: Unconjugated
• MW: 68.6
• Target: P2RX7 / P2X7 receptor
• Entrez: 5027
• Protein Sequence: YETNKVTRIQSMNY
• RRID: AB_10753748
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti P2RX7 antibody, anti purinergic receptor P2X,
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 37kDa and 70kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 68.6kDa according to NP_002553.2). Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Human Brain (Frontal Cortex) lysate using P2RX7 antibody

Immunohistochemical staining of Human Brain Cortex using P2RX7 antibody

Immunohistochemical staining of Human Kidney using P2RX7 antibody

orb20291 (3.75 µg/mL) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb20291 (0.3 µg/mL) staining of A431 (A) and HeLa (B) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

orb20291 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

orb20291 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.