p27 Antibody / p27Kip1

• Catalog Number: orb750185
• Category: Antibodies
• Description: This mAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPM348
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Mouse p27Kip1 protein was used as the immunogen for this p27 antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This p27Kip1 antibody is available for research use only.
• UniProt ID: P46527
• Storage: Store the p27Kip1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA fixed human MCF7 cells with p27Kip1 antibody (clone SPM348, green) and Phalloidin (red).

Western blot testing of human A) HeLa and B) Jurkat cell lysate using p27Kip1 antibody (clone SPM348).

IHC analysis of formalin-fixed, paraffin-embedded human prostate carcinoma stained with p27 antibody (clone SPM348).