p27 Antibody / p27Kip1

• Catalog Number: orb749446
• Category: Antibodies
• Description: This mAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This mAb is excellent for staining of formalin-fixed tissues.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: KIP1/769
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human protein was used as the immunogen for the p27Kip1 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This p27Kip1 antibody is available for research use only.
• UniProt ID: P46527
• Storage: Store the p27Kip1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the p27Kip1 antibody to be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with p27Kip1 antibody (clone KIP1/769).

IHC: Formalin-fixed, paraffin-embedded human prostate cancer stained with p27Kip1 antibody (clone KIP1/769).

IHC: Formalin-fixed, paraffin-embedded human cervical cancer stained with p27Kip1 (clone KIP1/769).

Flow cytometry testing of Jurkat cells. Black: cells alone; Grey: isotype control; Green: Alexa Fluor 488-labeled p27Kip1 antibody (clone KIP1/769).

IHC: Formalin-fixed, paraffin-embedded rat colon stained with p27Kip1 antibody (clone KIP1/769).

Immunofluorescent staining of PFA-fixed human MCF7 cells with p27Kip1 antibody (clone KIP1/769, green) and Phalloidin (red).

SDS-PAGE analysis of purified, BSA-free p27Kip1 antibody (clone KIP1/769) as confirmation of integrity and purity.