p27 antibody

• Catalog Number: orb1151679
• Category: Antibodies
• Description: Mouse monoclonal antibody to p27
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rKIP1/1356
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant full-length human CDKN1B/p27Kip1 protein was used as the immunogen for the recombinant p27Kip1 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant p27Kip1 antibody is available for research use only.
• UniProt ID: P46527
• Storage: Aliquot the recombinant p27Kip1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant p27Kip1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human breast carcinoma tissue with recombinant p27Kip1 antibody (clone rKIP1/1356). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant p27Kip1 antibody (clone rKIP1/1356) as confirmation of integrity and purity.