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Catalog Number | orb1294337 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to p21 Cip1 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rabbit |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / encompassing a sequence within the center region |
Dilution range | Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:400 Immunohistochemistry (Paraffin) 1:100–1:200 |
Conjugation | Unconjugated |
Target | p21 Waf1 / Cip1 |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
p21 Cip1 antibody at 1/500 dilution, Lysates at 30 μg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294337 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling p21 Cip1 with orb1294337 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling p21 Cip1 with orb1294337 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling p21 Cip1 with orb1294337 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling p21 Cip1 with orb1294337 at 1/100.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294337 Anti-p21 Cip1 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.
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