p21 Antibody / CDKN1A

• Catalog Number: orb749443
• Category: Antibodies
• Description: This mAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This mAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CIP1/823
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: Recombinant full-length human protein was used as the immunogen for the p21 antibody.
• Dilution range: Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This p21 antibody is available for research use only.
• UniProt ID: P38936
• Storage: Store the p21 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the p21 antibody to be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma (SCC) stained with p21 antibody (clone CIP1/823).

IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 antibody (clone CIP1/823).

Immunofluorescent staining of human HeLa cells with p21 antibody (red, clone CIP1/823) and Phalloiden (green).

Western blot testing of human HeLa cell lysate with p21 antibody (clone CIP1/823).