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Ovalbumin Antibody Peroxidase Conjugated

Catalog Number: orb344855

DispatchUsually dispatched within 5-10 working days
$ 700.00
Catalog Numberorb344855
CategoryAntibodies
DescriptionOvalbumin antibody (Peroxidase)
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, IHC, IP, WB
ReactivityGallus
IsotypeIgG
ImmunogenAnti-Ovalbumin Antibody was produced by repeated immunizations with hen egg white Ovalbumin protein.
Concentration1.0 mg/mL
Dilution rangeELISA: 1:5,000 - 1:25,000, IHC: 1:500 - 1:2,500, IP: 1:100, WB: 1:1,000 - 1:5,000
Form/AppearanceLyophilized
PurityOvalbumin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White]. Cross reactivity against Ovalbumin from other tissues and species may occur but have not been specifically determined.
ConjugationHRP
UniProt IDP01012
NCBIP01012.2
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Buffer/Preservatives0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Alternative namesrabbit anti-Ovalbumin Antibody Peroxidase Conjugat
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NoteFor research use only
Application notesAnti-Ovalbumin Antibody Peroxidase Conjugated has been tested by ELISA and western blot and is suitable for IHC and IP. Researchers should determine optimal titers for applications that are not stated below.
Expiration Date12 months from date of receipt.
Ovalbumin Antibody Peroxidase Conjugated

Western Blot of Rabbit anti-Ovalbumin Peroxidase Conjugated Antibody. Lane 1: Ovalbumin (Hen Egg). Load: 50 ng per lane. Primary antibody: Rabbit anti-Ovalbumin Peroxidase Conjugated Antibody at 1:1000 overnight at 4°C. Secondary antibody: none Block: orb348637 for 30 min at RT. Predicted/Observed size: 43 kDa, 43 kDa for Ovalbumin. Other band(s): Ovalbumin splice variants and isoforms.

Ovalbumin Antibody Peroxidase Conjugated

Western Blot of Rabbit anti-Ovalbumin Peroxidase Conjugated Antibody. Lane 1: Ovalbumin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Rabbit anti-Ovalbumin Peroxidase Conjugated Antibody at 1:1000 overnight at 4°C. Secondary antibody: none Block: orb348637 for 30 min at RT. Predicted/Observed size: 43 kDa, 43 kDa for Ovalbumin. Other band(s): Ovalbumin splice variants and isoforms.