OVALBUMIN Antibody

• Catalog Number: orb2652730
• Category: Antibodies
• Description: OVALBUMIN Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IP, WB
• Reactivity: Gallus
• Isotype: IgG
• Immunogen: Ovalbumin [Hen Egg White]
• Concentration: 10.0 mg/mL
• Dilution range: ELISA: 1:25,000, IHC: 1:300 - 1:2,000, IP: 1:100, WB: 1:500 - 1:2,000
• Form/Appearance: Lyophilized
• Purity: Rabbit Anti-Ovalbumin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White]. Cross reactivity against Ovalbumin from other sources is unknown.
• Conjugation: Unconjugated
• Hazard Information: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Biorbyt are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Biorbyt, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
• UniProt ID: P01012
• NCBI: P01012.2
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Alternative names: rabbit anti-Ovalbumin Antibody, Egg albumin antibo
• Note: For research use only
• Application notes: Anti-Ovalbumin Antibody has been tested in ELISA and Western Blot. Although not tested this product could be useful in assays such as IHC and IP. Specific conditions for reactivity and signal detection should be optimized by the end user. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 5.0 mL
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit anti-OVALBUMIN (Hen Egg White) antibody. Lane 1: Reduced Ovalbumin. Load: 50 ng. Primary antibody: Ovalbumin antibody at 1:1000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (p/n orb348637) for 30 min at RT. Predicted/Observed size: 45 kDa, 45 kDa for Ovalbumin.

Western Blot of Rabbit anti-Ovalbumin antibody. Lane 1: Ovalbumin protein - Reduced [~1 µg]. Lane 2: Ovalbumin protein - Reduced [0.25 µg]. Lane 3: Ovalbumin protein - Non-reduced [~1 µg]. Lane 4: Ovalbumin protein - Non-reduced [0.25 µg]. Primary antibody: Ovalbumin antibody at 1:5000 for overnight at 4°C. Secondary antibody: Atto 425 conjugated goat anti-rabbit secondary antibody at 1:10000 for 1.5 hr at RT. Block: orb348637 buffer overnight at 4°C. Predicted/Observed size: 42.9 kDa, ~36 kDa for ovalbumin. Other band(s): none.