Ovalbumin antibody (FITC)

• Catalog Number: orb344847
• Category: Antibodies
• Description: Ovalbumin antibody (FITC)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IF, IP, WB
• Reactivity: Gallus
• Isotype: IgG
• Immunogen: Ovalbumin [Hen Egg White]
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IF: 1:500 - 1:2,500, IP: 1:100, WB: 1:1,000 - 1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Ovalbumin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White]. Cross reactivity against Ovalbumin from other tissues and species may occur but have not been specifically determined.
• Conjugation: FITC
• UniProt ID: P01012
• NCBI: P01012.2
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Ovalbumin Antibody FITC Conjugation, f
• Note: For research use only
• Application notes: Anti-Ovalbumin fluorescein conjugated antibody has been tested by ELISA, dot blot, and western blot and is suitable for fluorescent western blot, immunofluorescence microscopy, and flow cytometry or FACS analysis as well as other antibody based fluorescent assays. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

Dot Blot of Rabbit anti-Ovalbumin (Hen Egg White) Fluorescein Conjugated. Antigen: Ovalbumin. Load: 100 ng, 33.3 ng, 11.1 ng, 3.7 ng, 1.23 ng as indicated. Primary antibody: N/A Secondary antibody: Rabbit anti-Ovalbulmin (Hen Egg White) antibody Fluorescein Conjugated 1:1000 for 60 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (orb348637) for 1 hour at RT.

Western Blot of Fluorescein conjugated Rabbit anti-Ovalbumin (Hen Egg white) antibody. Lane 1: Non-Reduced Ovalbumin. Lane 2: Opal Pre-stained Ladder. Lane 3: Reduced Ovalbumin. Load: 0.05 µg per lane. Primary antibody: none. Secondary antibody: Fluorescein Rabbit anti-Ovalbumin antibody at 1:1000 for 60 min at RT. Predicted/Observed size: ~35 kDa.

Western Blot of Rabbit anti-Ovalbumin antibody. Lane 1: ~1 µg Ovalbumin protein reduced. Lane 2: 0.25 µg Ovalbumin protein reduced. Lane 3: ~1 µg Ovalbumin protein non-reduced. Lane 4: 0.25 µg Ovalbumin protein non-reduced. Primary antibody: Ovalbumin antibody at 1:5000 for overnight at 4°C. Secondary antibody: Atto 425 conjugated goat anti rabbit secondary antibody at 1:10000 for 1.5 hr at RT. Block: orb348637 buffer overnight at 4°C. Predicted/Observed size: 42.9 kDa, ~36 kDa for ovalbumin. Other band(s): none.