Other PGE2 ELISA Kit

• Catalog Number: orb781723
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Prostaglandin E2(PGE2) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Prostaglandin E2(PGE2).
• Tested applications: ELISA
• Reactivity: All
• Range: 3.13-200 pg/mL
• Concentration: 2000 pg/mL
• Sensitivity: 0.97 pg/mL
• Protein Length: 2h
• Alternative names: PG-E2 ELISA Kit
• Note: For research use only
• Application notes: Standard: 200 pg/mL. Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PGE2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PGE2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PGE2 in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: serum, plasma and other biological fluids
• Expiration Date: Please enquire.