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| Catalog Number | orb781703 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Nitrotyrosine(NT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Nitrotyrosine(NT). |
| Tested applications | ELISA |
| Reactivity | All |
| Range | 4.69-300 ng/mL |
| Concentration | 300 ng/mL |
| Sensitivity | 1.4 ng/mL |
| Protein Length | 2h |
| Alternative names | 3-Nitro-L-Tyrosine ELISA Kit, 3-Nitrotyrosine ELIS Read more... |
| Note | For research use only |
| Application notes | standard: 300 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 3-NT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 3-NT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 3-NT in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma and other biological fluids |
| Expiration Date | Please enquire. |
