You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb774907 |
---|---|
Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Bovine Serum Albumin(BSA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Bovine Serum Albumin(BSA). |
Tested applications | ELISA |
Reactivity | All |
Range | 468.75-30000 ng/mL |
Concentration | 30000 ng/mL |
Sensitivity | 167.8 ng/mL |
Protein Length | 2h |
Alternative names | Bovine Albumin ELISA Kit, Fraction V ELISA Kit Read more... |
Note | For research use only |
Application notes | standard: 30000 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with BSA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to BSA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BSA in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Expiration Date | Please enquire. |