You have no items in your shopping cart.
Cart summary
| Catalog Number | orb781667 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Hydroxytryptamine(5-HT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Hydroxytryptamine(5-HT). |
| Tested applications | ELISA |
| Reactivity | All |
| Range | 14.07-900 ng/mL |
| Concentration | 900 ng/mL |
| Sensitivity | 3.94 ng/mL |
| Protein Length | 2h |
| Alternative names | 5HT ELISA Kit, Serotonin ELISA Kit, N-Methyl-Gamma Read more... |
| Note | For research use only |
| Application notes | standard: 900 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-HT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum and plasma |
| Expiration Date | Please enquire. |
