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Catalog Number | orb1958343 |
---|---|
Category | Proteins |
Description | Osteopontin Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 35 kDa and the accession number is A0A024RDE2. |
Tag | C-His |
Purity | 91.60% |
MW | 35 kDa (predicted); 60-65 kDa (reducing condition, due to glycosylation) |
UniProt ID | P10451 |
Expression System | HEK293 Cells |
Biological Origin | Human |
Biological Activity | Osteopontin Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 35 kDa and the accession number is A0A024RDE2. |
Expression Region | A DNA sequence encoding the pro form of human SPP1 (NP_001035147.1) (Met 1-Asn 314) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Ile 17 |
Storage | -20°C |
Note | For research use only |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
90% | |
33.0 kDa | |
Human Osteopontin, His Tag (orb257724) is expressed from human 293 cells (HEK293). It contains AA Ile 17 - Asn 300 (Accession # AAH07016). |
> 90% as determined by SDS-PAGE | |
34.8 kDa |
98.00% | |
34.8 kDa (predicted). Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. |
95.30% | |
The recombinant human SPP1 consists of 185 amino acids and predicts a molecular mass of 20.64 kDa. |
SDS-PAGE: 94.9%; SEC-HPLC: 91.6% | |
193.32 kDa (predicted) |