ORC3L Antibody

• Catalog Number: orb1247172
• Category: Antibodies
• Description: ORC3L Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: QKTDHVARLTWGGC
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 82.3kDa according to NP_862820.1 and NP_036513.2). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ORC3L
• UniProt ID: Q9UBD5
• NCBI: NP_036513.2, NP_862820.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ORC3L, origin recognition complex, subunit 3-like
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 82.3kDa according to NP_862820.1 and NP_036513.2). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247172 (2 ug/ml) staining of Hela lysate (RIPA buffer, 1.4E5 cells per lane). Detected by chemiluminescence.

orb1247172 (3.75 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.