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Catalog Number | orb1239693 |
---|---|
Category | Antibodies |
Description | ORAI1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Predicted Reactivity | Gallus, Rat |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | ORAI1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ORAI1.The immunogen is located within the last 50 amino acids of ORAI1. |
Concentration | 1 mg/mL |
Dilution range | ORAI1 antibody can be used for detection of ORAI1 by Western blot at 0.5 - 2 μg/mL. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | ORAI1 |
UniProt ID | Q96D31 |
NCBI | Q96D31 |
Storage | ORAI1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | ORAI1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | ORAI1 Antibody: IMD9, ORAT1, CRACM1, TMEM142A, Cal Read more... |
Note | For research use only |
Application notes | ORAI1 antibody can be used for detection of ORAI1 by Western blot at 0.5 - 2 μg/mL. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at (A) 0.5, (B) 1 and (C) 2 µg/mL.
Immunohistochemistry of ORAI1 in human ovary tissue with ORAI1 antibody at 2.5 µg/ml.
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 2.5 µg/mL.
Immunofluorescence of ORAI1 in human ovary tissue with ORAI1 antibody at 20 µg/mL. Red: ORAI1 Antibody (orb1239693) Blue: DAPI staining.
ELISA, IF, IHC-P, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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