OPG Rabbit Polyclonal Antibody

• Catalog Number: orb312402
• Category: Antibodies
• Description: OPG Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human OPG (221-300/401aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 43 kDa
• Target: TNFRSF11B
• UniProt ID: O00300
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: MGC29565; OCIF; OPG; Osteoclastogenesis Inhibitory
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Liu, Tong-Jun et al. Overexpression of microRNA-141 inhibits osteoporosis in the jawbones of ovariectomized rats by regulating the Wnt/β-catenin pathway Arch. Oral Biol., 113, 104713 (2020)

PubMed: 32229339

Applications

IHC

Reactivity

Rat

Sample: Heart (Mouse) Lysate at 40 ug, Kidney (Mouse) Lysate at 40 ug, Liver (Mouse) Lysate at 40 ug, Primary: Anti-OPG (orb312402) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43kD, Observed band size: 50kD.

Sample: Lane 1: Raji (Human) Cell Lysate at 30 ug, Lane 2: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-OPG (orb312402) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 55 kD.