OLIG2 antibody

• Catalog Number: orb536865
• Category: Antibodies
• Description: Rabbit polyclonal antibody to OLIG2
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-80 of human Olig2 (NP_005797.1).
• Dilution range: WB: 1:500-1:2000, IHC-P: 1:50-1:100
• Conjugation: Unconjugated
• MW: 35kDa
• UniProt ID: Q13516
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
• Alternative names: OLIG2, BHLHB1, OLIGO2, PRKCBP2, RACK17, bHLHe19
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis of paraffin-embedded mouse brain using Olig2 Rabbit pAb (orb536865) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat brain using Olig2 Rabbit pAb (orb536865) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Western blot analysis of various lysates using Olig2 Rabbit pAb (orb536865) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.