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Catalog Number | orb1929704 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB21522 |
Tested applications | WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:2000 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 38782 Da |
Target | This OGG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 318-345 amino acids from the C-terminal region of human OGG1. |
UniProt ID | O15527 |
NCBI | NP_002533.1, NP_058438.1, NP_058213.1, NP_058437.1, NP_058434.1, NP_058436.1, NP_058214.1, NP_058212.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | N-glycosylase/DNA lyase, 8-oxoguanine DNA glycosyl Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of OGG1 Antibody (C-term) in Hela (lane 1), NIH-3T3 (lane 2) cell line lysates (35 ug/lane). OGG1 (arrow) was detected using the purified Pab.
All lanes: Anti-OGG1 Antibody (C-term) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: HL-60 whole cell lysate. Lane 4: Jurkat whole cell lysate. Lane 5: NCCIT whole cell lysate. Lane 6: Raji whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 39 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.