Oct4 Antibody / POU5F1

• Catalog Number: orb1716300
• Category: Antibodies
• Description: OCT4/POU5F1 is a member of the POU family of transcription factors, involved in the regulation of pluripotency during normal development and is detectable in embryonic stem and germ cells. It is a key regulator of self-renewal in embryonic stem cells; its expression is potentially correlated with tumorigenesis and can affect tumor recurrence or resistance to therapies. OCT4/POU5F1 is expressed in undifferentiated pluripotent cells, germ cells in ovary and testis.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: OCT4/6847R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Recombinant fragment from the human protein was used as the immunogen for the POU5F1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This POU5F1 antibody is available for research use only.
• UniProt ID: Q01860
• Storage: Aliquot the POU5F1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the POU5F1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human seminoma tissue with OCT4 antibody (clone OCT4/6847R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human seminoma tissue with OCT4 antibody (clone OCT4/6847R) at 2 ug/ml. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.