Oct4 Antibody / POU5F1

• Catalog Number: orb1151467
• Category: Antibodies
• Description: Rabbit monoclonal antibody to OCT4
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: OCT4/6875R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A portion of amino acids1-134 was used as the immunogen for the recombinant Oct4 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant Oct4 antibody is available for research use only.
• UniProt ID: Q01860
• Storage: Aliquot the recombinant antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human testis with recombinant Oct4 antibody (clone OCT4/6875R). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human seminoma tissue with recombinant Oct4 antibody (clone OCT4/6875R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human seminoma tissue with recombinant Oct4 antibody (clone OCT4/6875R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.