Occludin antibody

• Catalog Number: orb2276304
• Category: Antibodies
• Description: Occludin antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Biorbyt-415M
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: ecombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary)
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: Q16625
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: BLCPMG; Occludin; OCLN; Phosphatase 1 regulatory s
• Note: For research use only
• Application notes: Positive Control: Colon: A strong membranous occludin staining of epithelial cells should be seen with a particularly strong staining at the apical/luminal membranes. Negative Control: Colon: All non-epithelial and non-endothelial cells must not show any occludin staining. Cellular Localization: Cell Surface and Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Clear cell ovarian carcinoma with moderate occludin staining of tumor cells.

In squamous epithelium occludin positivity is regularly seen but staining is reduced or absent in basal and superficial cell layers scaled.

Membranous occludin immunostaining of respiratory epithelium scaled.