Occludin antibody

• Catalog Number: orb1294255
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Occludin
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human Occludin / OCLN
• Dilution range: Western Blot 1:500-1:1500 Immunofluorescence 1:200–1:300 Immunohistochemistry 1:100
• Conjugation: Unconjugated
• Target: Occludin
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-Occludin antibody - at 1/1000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294255 overnight at 4°C.

Anti-Occludin antibody - at 1/1000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294255 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling Occlidin with orb1294255 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling Occlidin with orb1294255 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.