OAT1 / SLC22A6 Rabbit Polyclonal Antibody

• Catalog Number: orb500999
• Category: Antibodies
• Description: OAT1 / SLC22A6 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human OCT-1 (285-550/550aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 62 kDa
• Target: SLC22A6
• UniProt ID: Q4U2R8
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Organic anion transporter 1; OAT1; OAT-1; solute c
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Kidney (Human) lysate at 30 ug, Brain (Rat) lysate at 30 ug, Primary: Anti-OAT-1/SLC22A6 (human) (orb500999) at 1:200 dilution, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000 dilution, Predicted band size: 62kD, Observed band size: 61kD.

Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-SLC22A6 (orb500999) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kD, Observed band size: 62 kD.

Sample: Raji Cell lysate at 30 ug, Primary: Anti-SLC22A6 (orb500999) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 62 kD, Observed band size: 62 kD.

Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-OAT-1/SLC22A6 (human) Polyclonal Antibody, Unconjugated (orb500999) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.