O-GalNAc Antibody: Biotin

• Catalog Number: orb396453
• Category: Antibodies
• Description: Mouse monoclonal antibody against O-GalNAC conjugated to Biotin.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 9B9
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: All
• Isotype: IgM
• Immunogen: Synthetic β-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH).
• Concentration: < p>1 mg/ml

• Dilution range: WB: 1:1000, IF/ICC: 1:50, ELISA: 1:1000, IHC-P: 1:25
• Conjugation: Biotin
• Target: O-GalNAC
• Storage: -20ºC, Conjugated antibodies should be stored at 4ºC
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
• Alternative names: GalNAC antibody, O-GalNAC antibody, β-O-Linked N-A
• Note: For research use only
• Application notes: A 1:10,000 dilution of SMC-503 was sufficient for detection of O-GalNAc in 2 µg of O-GalNAc conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GalNAc Antibody (D) Composite.

Immunohistochemistry analysis using Mouse Anti-O-GalNAc Monoclonal Antibody, Clone 9B9. Tissue: colon. Species: Mouse. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-O-GalNAc Monoclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GalNAc Antibody (D) Composite.

Western Blot analysis of Glycoconjugates showing detection of 67 kDa O-GalNAC protein using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9. Lane 1: Molecular Weight Ladder (MW). Lane 2: GlcNAc-BSA. Lane 3: GalNAc-BSA. Lane 4: Galactose-BSA. Lane 5: Glucose-BSA. Load: 2.0 μg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.