Nucleolin Antibody

• Catalog Number: orb385928
• Category: Antibodies
• Description: Nucleolin (NCL) is the major nucleolar phosphoprotein of growing eukaryotic cells. It is located mainly in dense fibrillar regions of the nucleolus. NCL is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: NPC23-2
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Mouse IgG1
• Immunogen: Recombinant human protein was used as the immunogen for the Nucleolin antibody.
• Dilution range: Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells in 0.1ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Nucleolin antibody is available for research use only.
• UniProt ID: P19338
• Storage: Store the Nucleolin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the Nucleolin antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human tonsil with Nucleolin antibody (clone NPC23-2). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

Immunofluorescent staining of PFA-fixed human K562 cells with Nucleolin antibody (green, clone NPC23-2) and Phalloidin (red).

Flow cytometry testing of human 293 cells with isotype control and Alexa Fluor 488-labeled Nucleolin antibody (green, clone NPC23-2).

Western blot testing of Nucleolin antibody (clone NPC23-2) and A431 lysate. Predicted molecular weight ~77 kDa.