Nucleolin Antibody

SKU: orb385928

Description

Nucleolin (NCL) is the major nucleolar phosphoprotein of growing eukaryotic cells. It is located mainly in dense fibrillar regions of the nucleolus. NCL is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.

Research Area

Epigenetics

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells in 0.1ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Reactivity: Human, Mouse
• Application Notes:
  Titering of the Nucleolin antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: NPC23-2
• Immunogen: Recombinant human protein was used as the immunogen for the Nucleolin antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human tonsil with Nucleolin antibody (clone NPC23-2). FFPE testing requires sections to be boiled in pH9 10 mM Tris with 1 mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

Immunofluorescent staining of PFA-fixed human K562 cells with Nucleolin antibody (green, clone NPC23-2) and Phalloidin (red).

Flow cytometry testing of human 293 cells with isotype control and Alexa Fluor 488-labeled Nucleolin antibody (green, clone NPC23-2).

Western blot testing of Nucleolin antibody (clone NPC23-2) and A431 lysate. Predicted molecular weight ~77 kDa.

Quick Database Links

UniProt

P19338