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Catalog Number | orb420341 |
---|---|
Category | Antibodies |
Description | Nuclear receptor ROR gamma (phospho-S203) antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Mouse |
Isotype | IgG |
Immunogen | Affinity purified Anti-receptor ROR gamma pS203 antibody was prepared from whole rabbit serum produced by repeated immunizations with an internal synthetic peptide phosphorylated at the serine 203 position of mouse receptor ROR gamma protein. |
Concentration | 1.05 mg/ml |
Dilution range | ELISA: 5 µg/ml, WB: 1 ug/mL |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-nuclear receptor ROR gamma pS203 is directed against mouse receptor ROR gamma phosphorylated at the serine 203 position. This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide to remove any unwanted reactivities. A BLAST analysis was used to suggest reactivity with this protein with pongo abelii based on 100% homology for the immunogen sequence. |
Conjugation | Unconjugated |
UniProt ID | P51450 |
NCBI | NP_001280663.1 |
Storage | Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-receptor ROR gamma pS203 antibody, Nuc Read more... |
Note | For research use only |
Application notes | This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 61 kDa in size corresponding to receptor ROR gamma by western blotting in the appropriate cell lysate or extract. |
Expiration Date | 12 months from date of receipt. |
Western Blot of Rabbit anti-receptor ROR gamma pS203 antibody. Lane 1: Opal PreStained Ladder. Lane 2: Wild-type Mouse Thymus. Lane 3: KO Mouse Thymus. Load: 10 µg per lane. Primary antibody: Receptor ROR gamma pS203 antibody at 1 ug/ml for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: (p/n orb348637) for 30 minutes at RT. Predicted/Observed size: 63 kDa for ROR gamma.
ELISA, WB | |
Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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