NT5E Antibody

• Catalog Number: orb1258329
• Category: Antibodies
• Description: NT5E Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 290-550 of human NT5E / CD73 (NP_002517.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 69kDa/75kDa
• Target: NT5E
• UniProt ID: P21589
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: NT5E, 5-nucleotidase, ecto (CD73), 5'-nucleotidase
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using NT5E/CD73 antibody (orb1258329) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human liver using NT5E/CD73 antibody (orb1258329) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using NT5E/CD73 antibody (orb1258329) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat testis using NT5E/CD73 antibody (orb1258329) at dilution of 1:100 (40x lens).