NSE gamma antibody

• Catalog Number: orb2276527
• Category: Antibodies
• Description: NSE gamma antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Biorbyt-451M
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: A synthetic peptide of human NSE gamma (around aa416-433) (exact sequence is proprietary)
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: P09104
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: ENO2; ENOG; Enolase 2 gamma neuronal; Enolase2; Ga
• Note: For research use only
• Application notes: Positive Control: In the colon, axons and ganglion cells in lamina propria and muscular wall must show at least a moderate NSE staining, while epithelial and lymphatic cells remain negative. Negative Control: In the colon, epithelial and lymphatic cells do not show NSE staining. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

A strong NSE immunostaining is seen in the granule cell layer and in the fibres of the white matter of the cerebellum.

Positive NSE immunostaining of islets of Langerhans.

Strong NSE immunostaining in an adrenal pheochromocytoma.