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NSE Antibody / Neuron Specific Enolase

Catalog Number: orb750096

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb750096
CategoryAntibodies
DescriptionThis mAb recognizes a protein of about 50 kDa which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
Species/HostMouse
ClonalityMonoclonal
Clone NumberENO2/1462
Tested applicationsIHC-P
ReactivityHuman, Mouse, Rat
IsotypeMouse IgG2b, kappa
ImmunogenAmino acids 416-433 of human NSE were used as the immunogen for this Neuron Specific Enolase antibody.
Dilution rangeImmunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis Neuron Specific Enolase antibody is available for research use only.
UniProt IDP09104
StorageStore the Neuron Specific Enolase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Neuron Specific Enolase antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
NSE Antibody / Neuron Specific Enolase

Protein array validation of the Neuron Specific Enolase antibody: Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using Neuron Specific Enolase antibody (clone ENO2/1462). These results demonstrate the foremost specificity of the ENO2/1462 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

NSE Antibody / Neuron Specific Enolase

IHC testing of FFPE human pheochromocytoma with Neuron Specific Enolase antibody (clone ENO2/1462). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.

NSE Antibody / Neuron Specific Enolase

IHC testing of FFPE mouse pancreas with Neuron Specific Enolase antibody (clone ENO2/1462). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.

NSE Antibody / Neuron Specific Enolase

IHC testing of FFPE rat heart with Neuron Specific Enolase antibody (clone ENO2/1462). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.

NSE Antibody / Neuron Specific Enolase

SDS-PAGE Analysis of Purified, BSA-Free Neuron Specific Enolase Antibody (clone ENO2/1462). Confirmation of Integrity and Purity of the Antibody.

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    IHC-P,  WB

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