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NSE Antibody / Neuron Specific Enolase

Catalog Number: orb386134

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb386134
CategoryAntibodies
DescriptionRecognizes a protein of about 50kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
Species/HostMouse
ClonalityMonoclonal
Clone NumberENO2/1375
Tested applicationsIHC-P, WB
ReactivityHuman, Mouse, Rat
IsotypeMouse IgG2b, kappa
ImmunogenAmino acids 416-433 of human Neuron Specific Enolase were used as the immunogen for this NSE antibody.
Dilution rangeWestern blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.2-0.4ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis NSE antibody is available for research use only.
Entrez2026
UniProt IDP09104
StorageStore the NSE antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance.
Expiration Date12 months from date of receipt.
NSE Antibody / Neuron Specific Enolase

IHC testing of FFPE human cerebellum with NSE antibody (clone ENO2/1375). Required HIER: boil sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

NSE Antibody / Neuron Specific Enolase

IHC testing of FFPE human pheochromocytoma with NSE antibody (clone ENO2/1375). Required HIER: boil sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

NSE Antibody / Neuron Specific Enolase

Western blot testing of human 1) Y79, 2) HeLa and 3) HepG2 cell lysate with NSE antibody (clone ENO2/1375). Predicted molecular weight ~47 kDa.

NSE Antibody / Neuron Specific Enolase

IHC testing of FFPE mouse pancreas with NSE antibody (clone ENO2/1375). Required HIER: boil sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

NSE Antibody / Neuron Specific Enolase

IHC testing of FFPE rat heart with NSE antibody (clone ENO2/1375). Required HIER: boil sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

NSE Antibody / Neuron Specific Enolase

SDS-PAGE Analysis of Purified, BSA-Free NSE Antibody (clone ENO2/1375). Confirmation of Integrity and Purity of the Antibody.

  • NSE Antibody / Neuron Specific Enolase [orb2640228]

    IHC-P,  WB

    Human, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    100 μg
  • NSE Antibody / Neuron Specific Enolase [orb750096]

    IHC-P

    Human, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    20 μg
  • NSE Antibody / Neuron Specific Enolase [orb2640394]

    IHC-P

    Human, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    100 μg
  • NSE Antibody / Neuron Specific Enolase [orb1825910]

    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg
  • NSE Antibody / Neuron Specific Enolase [orb1825911]

    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg