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Catalog Number | orb386134 |
---|---|
Category | Antibodies |
Description | Recognizes a protein of about 50kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | ENO2/1375 |
Tested applications | IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Mouse IgG2b, kappa |
Immunogen | Amino acids 416-433 of human Neuron Specific Enolase were used as the immunogen for this NSE antibody. |
Dilution range | Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.2-0.4ug/ml for 30 min at RT |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This NSE antibody is available for research use only. |
Entrez | 2026 |
UniProt ID | P09104 |
Storage | Store the NSE antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance. |
Expiration Date | 12 months from date of receipt. |
IHC testing of FFPE human cerebellum with NSE antibody (clone ENO2/1375). Required HIER: boil sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.
IHC testing of FFPE human pheochromocytoma with NSE antibody (clone ENO2/1375). Required HIER: boil sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.
Western blot testing of human 1) Y79, 2) HeLa and 3) HepG2 cell lysate with NSE antibody (clone ENO2/1375). Predicted molecular weight ~47 kDa.
IHC testing of FFPE mouse pancreas with NSE antibody (clone ENO2/1375). Required HIER: boil sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.
IHC testing of FFPE rat heart with NSE antibody (clone ENO2/1375). Required HIER: boil sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.
SDS-PAGE Analysis of Purified, BSA-Free NSE Antibody (clone ENO2/1375). Confirmation of Integrity and Purity of the Antibody.
IHC-P, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |