NRF1 Rabbit Polyclonal Antibody

• Catalog Number: orb575133
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NRF1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Rabbit, Rat, Zebrafish
• Reactivity: Human, Mouse
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human NRF1
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 54 kDa
• Target: NRF1
• UniProt ID: Q16656
• Protein Sequence: Synthetic peptide located within the following region: WATLQGGEMTIQTTQASEATQAVASLAEAAVAASQEMQQGATVTMALNSE
• NCBI: NP_005002
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: ALPHA-PAL
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/ml of the antibody was used in this experiment.

Sample Tissue: Mouse Heart, Antibody dilution: 1 ug/ml.

Sample Tissue: Human A549 Whole Cell, Antibody dilution: 3 ug/ml.

Sample Tissue: Human U937 Whole Cell, Antibody dilution: 2 ug/ml.

Human 293T. There is BioGPS gene expression data showing that NRF1 is expressed in HEK293T.

Human Hela. There is BioGPS gene expression data showing that NRF1 is expressed in HeLa.

Human Jurkat. There is BioGPS gene expression data showing that NRF1 is expressed in Jurkat.

Human Prostate

Rabbit Anti-NRF1 Antibody, Catalog Number: orb575133, Formalin Fixed Paraffin Embedded Tissue: Human Adult heart, Observed Staining: Nuclear, Primary Antibody Concentration: 1:600, Secondary Antibody: Donkey anti-Rabbit-Cy2/3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5- 2.0 sec.

Rabbit Anti-NRF1 Antibody, Catalog Number: orb575133, Formalin Fixed Paraffin Embedded Tissue: Human Adult liver, Observed Staining: Nuclear and some weak cytoplasmic, Primary Antibody Concentration: 1:600, Secondary Antibody: Donkey anti-Rabbit-Cy2/3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5 -2.0 sec.

WB Suggested Anti-NRF1 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:62500, Positive Control: Transfected 293T.

WB Suggested Anti-NRF1 antibody Titration: 1 ug/ml, Sample Type: Human heart.