NRAS Antibody

• Catalog Number: orb1247186
• Category: Antibodies
• Description: NRAS Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine, Mouse
• Reactivity: Human, Rat
• Immunogen: The immunogen for this antibody is: C-RMKKLNSSDDGTQ
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 21kDa band observed in Human lysates of cell line A431 and of Rat Intestines (calculated MW of 21.2kDa according to NP_002515.1). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on NIH3T3 for this particular batch.Immunohistochemistry:In paraffin embedded Human Small Intestine shows staining of cytoplasm and membrane of enterocytes. Recommended concentration: 2-3ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NRAS
• UniProt ID: P01111
• NCBI: NP_002515.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: NRAS , neuroblastoma RAS viral (v-ras) oncogene ho
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 21kDa band observed in Human lysates of cell line A431 and of Rat Intestines (calculated MW of 21.2kDa according to NP_002515.1). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on NIH3T3 for this particular batch.Immunohistochemistry:In paraffin embedded Human Small Intestine shows staining of cytoplasm and membrane of enterocytes. Recommended concentration: 2-3ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247186 (2.5 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247186 (0.01 ug/ml) staining of A431 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247186 (0.1 ug/ml) staining of Rat Intestines lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.