NOTCH1 antibody

• Catalog Number: orb373660
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NOTCH1, which is also known as AOVD. NOTCH1 is frequently found in the intracellular domain of the nucleus and is expressed in the basal keratinocytes and mesencephalic ventricular zone of the neural tube. It is overexpressed in the cerebrospinal fluid. This protein extensively functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus.
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein of human Notch1(NP_060087.3).
• Dilution range: WB: 1:500-2000, IHC-P: 1:100-200
• Conjugation: Unconjugated
• MW: 300KDa
• UniProt ID: P46531
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Alternative names: AOS5, AOVD1, TAN1, hN1, NOTCH1, Activated NOTCH1,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of human esophagus tissue using NOTCH1 antibody

Western blot analysis of extracts of HT-29 cells, using NOTCH1 Antibody