NOTCH1 Antibody

• Catalog Number: orb1672173
• Category: Antibodies
• Description: NOTCH1 Antibody
• Clonality: Recombinant
• Clone Number: E6
• Tested applications: ELISA, IHC
• Reactivity: Human, Mouse
• Isotype: IgG lambda
• Immunogen: This antibody was selected against a fusion protein consisting of EGF domains 1–12 of murine Notch1 fused to a human Fc domain and binds to to the NRR of mouse Notch1.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: NOTCH1
• UniProt ID: P46531, Q01705
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Tan1, Notch1, Mis6, N1, lin-12, NOTCH1, TAN1, hN1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the anti-Notch 1 antibody E6. Mouse thymocytes were stained with unimmunized r antibody (black line) or the rmeric version of E6 (orb1672173 - blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.

Immunofluoresence staining of fixed HeLa cells with anti-Notch 1 antibody E6. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton stained with the chimeric r version of E6 (orb1672173) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672173- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor® 488 secondary antibody.