NOD2 antibody

• Catalog Number: orb1474649
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NOD2
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein corresponding to human NOD2. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/200)
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: NOD2
• Entrez: 64127
• UniProt ID: Q9HC29, Q8K3Z0
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: CARD15; IBD1; Nucleotide-binding oligomerization d
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NOD2 expression in mouse kidney (A), rat spleen (B) whole cell lysates. (Predicted band size: 22; 112; 115 kD; Observed band size: 100 kD)

Immunohistochemical analysis of NOD2 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.