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Catalog Number | orb507553 |
---|---|
Category | Antibodies |
Description | nmt55 p54nrb NONO Antibody (monoclonal, 11E2) |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 11E2 |
Tested applications | FC, ICC, IF, WB |
Reactivity | Human |
Isotype | Mouse IgG1 |
Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human nmt55 p54nrb (1-35aa MQSNKTFNLEKQNHTPRKHHQHHHQQQHHQQQQQQ), identical to the related mouse and rat sequences. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 60 kDa |
UniProt ID | Q15233 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Non-POU domain-containing octamer-binding protein; Read more... |
Note | For research use only |
Application notes | Western blot, 0.1-0.5μg/ml Immunocytochemistry, 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 5μg/ml Flow Cytometry, 1-3μg/1x10^6 cells |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U20S cells using anti-nmt55 p54nrb antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of nmt55 p54nrb using anti-nmt55 p54nrb antibody.Lane 1:human HELA cell; 2:human placenta tissue; 3:human MCF-7 cell; 4:human A549 cell; 5:human SW620 cell; 6:human PANC-1 cell; 7:human U20S cell; 8:human K562 cell.
IF analysis of nmt55 p54nrb using anti-nmt55 p54nrb antibody. nmt55 p54nrb was detected in immunocytochemical section of MCF-7 cells.
IHC analysis of nmt55 p54nrb using anti-nmt55 p54nrb antibody.nmt55 p54nrb was detected in immunocytochemical section of A431 cell.
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