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Catalog Number | orb1151877 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to NKX3.1 |
Species/Host | Mouse |
Clonality | Recombinant |
Clone Number | rNKX3.1/6620 |
Tested applications | ELISA, IHC-P |
Reactivity | Human |
Isotype | Mouse IgG1, kappa |
Immunogen | A portion of amino acids 92-224 was used as the immunogen for the NKX3.1 antibody. |
Dilution range | ELISA (For coating order BSA-free format),Immunohistochemistry (FFPE): 1-2ug/ml |
Purity | Protein A/G affinity |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This NKX3.1 antibody is available for research use only. |
UniProt ID | Q99801 |
Storage | Aliquot the NKX3.1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the NKX3.1 antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human prostate tissue with NKX3.1 antibody (clone rNKX3.1/6620). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue using NKX3.1 antibody (clone rNKX3.1/6620) at 2 ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free NKX3.1 antibody (rNKX3.1/6620) as confirmation of integrity and purity.
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