NKX2.2 Antibody

• Catalog Number: orb322972
• Category: Antibodies
• Description: NKX2.2 acts as a transcriptional activator. Required for the maintenance of NEUROD1 expression in the horomone-producing endocrine cells of the pancreas. May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Associates with chromatin at the NEUROD1 promoter region. Binds to a subset of consensus elements within the NEUROD1 promoter (By similarity). [UniProt]
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: RMNK2-1
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A human partial recombinant protein was used as the immunogen for this recombinant NKX2.2 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant NKX2.2 antibody is available for research use only.
• Entrez: 4821
• Storage: Store the recombinant NKX2.2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the recombinant NKX2.2 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human pancreas with recombinant NKX2.2 antibody (clone RMNK2-1). HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.

IHC testing of FFPE Ewings sarcoma with recombinant NKX2.2 antibody (clone RMNK2-1). HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.