Nitrotyrosine antibody (Biotin)

• Catalog Number: orb1108779
• Category: Antibodies
• Description: Mouse monoclonal antibody against Nitrotyrosine conjugated to Biotin
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IA, IHC-Fr, PS, WB
• Isotype: Mouse IgG2b
• Form/Appearance: 0.5 ml (100 µg/ml) 0.2 um filtered protein G purified biotinylated antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
• Conjugation: Biotin
• Storage: Product should be stored at 4degC. Under recommended storage conditions, product is stable for one year.
• Note: For research use only
• Application notes: F: also cytospins: acetone fixation 10 min -20 degC; block endogenous peroxidase by 0.3 % H2O2 in PBS (or methanol for intracellular staining); blocking with 10% NGS or 5 % BSA for 30 min. use at assay dependent concentration; P:10% formalin fixation; 3% H2O2 to block endogenous peroxidases; Citrate buffer pH 6.0 for 1 min at 100 degC as antigen retrieval treatment; use at assay dependent concentration (1:200/400)- (Ref 1); W: reduced and no-reduced samples; block with 5% BSA or skimmed milk, use at assay dependent concentration
• Expiration Date: 12 months from date of receipt.