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Catalog Number | orb769737 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Nicalin |
Clonality | Polyclonal |
Tested applications | ELISA, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from human NCLN. AA range:474-523 |
Concentration | 1 mg/ml |
Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
Conjugation | Unconjugated |
MW | 63 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-NCLN antibody, anti-Nicalin antibody, anti-Ni Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Western blot analysis of lysates from HepG2 and HUVEC cells, using NCLN Antibody. The lane on the right is blocked with the synthesized peptide.
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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