NGF Receptor Antibody / p75NTR / CD271

• Catalog Number: orb639828
• Category: Antibodies
• Description: It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR is a reliable marker for desmoplastic and neurotropic melanomas. NGFR is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rNGFR/1965
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A recombinant human partial protein (amino acids 281-421) was used as the immunogen for this recombinant NGF Receptor antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml (not suitable for frozen sample testing),Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant NGF Receptor antibody is available for research use only.
• UniProt ID: P08138
• Storage: Store the recombinant NGF Receptor antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human U-87 cell lysate with recombinant NGF Receptor antibody (clone rNGFR/1965). Predicted molecular weight: 45-75 kDa depending on glycosylation level.

IHC staining of FFPE human melanoma with recombinant NGF Receptor antibody (clone rNGFR/1965). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human melanoma with recombinant NGF Receptor antibody (clone rNGFR/1965). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant NGF Receptor antibody (clone rNGFR/1965) as confirmation of integrity and purity.