NFKB1 antibody

• Catalog Number: orb216169
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NF-kappaB p105 (Phospho-S932)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S932 of human NF-kappaB p105 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: NFKB1
• Entrez: 18033, 4790
• UniProt ID: P19838, P25799, Q63369
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti Nuclear factor NF-kappa-B p105 subunit antibo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NF-kappaB p105 (Phospho-S932) expression in HeLa LPS-treated (A), NIH3T3 LPS-treated (B), Raw264.7 LPS-treated (C), rat kidney (D) whole cell lysates. (Predicted band size: 105 kD; Observed band size: 105 kD)

Immunohistochemical analysis of NF-kappaB p105 (Phospho-S932) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.